GLOW Dosage & Reconstitution Guide
⚠ Research compound, not for human use. Avoid with active or prior cancer due to angiogenic activity; avoid in pregnancy and breastfeeding.
Research and educational use only — not medical advice, and not for human consumption. The figures below are summarized from public sources, product labeling, and community reports, and are not a prescription or a substitute for a qualified clinician.
Reconstitution
GLOW ships as a freeze-dried (lyophilized) powder that has to be mixed with bacteriostatic water before use. Draw 2 mL of bacteriostatic water into a syringe and add it to a 70 mg vial, letting the stream run down the inside wall of the glass rather than blasting the powder directly. Swirl gently — never shake — until the solution is clear, then keep it refrigerated.
Worked example. 2 mL into a 70 mg vial gives a concentration of 35 mg/mL (35000 mcg/mL). A 3.5 mg dose then works out to 0.1 mL = 10 units on a U-100 insulin syringe. Scale the water volume up or down to make your target dose land on an easy-to-read mark (1 unit = 0.01 mL).
- Wipe both vial stoppers (peptide and bacteriostatic water) with an alcohol swab and let them dry.
- Draw 2 mL of bacteriostatic water into a syringe.
- Inject it slowly against the inside wall of the peptide vial.
- Swirl gently until fully dissolved; do not shake.
- Label the vial with the contents, concentration, and date, and store it in the refrigerator.
Dosing schedule
| Phase | Dose | Volume | Insulin units (U-100) | Notes |
|---|---|---|---|---|
| Low / starting (5 units) | 1.75 mg | 0.05 mL | 5 units | ~1.25 mg GHK-Cu + 0.25 mg BPC-157 + 0.25 mg TB-500 |
| Standard (10 units) | 3.5 mg | 0.1 mL | 10 units | ~2.5 mg GHK-Cu + 0.5 mg BPC-157 + 0.5 mg TB-500 |
| Moderate (15 units) | 5.25 mg | 0.15 mL | 15 units | ~3.75 mg GHK-Cu + 0.75 mg BPC-157 + 0.75 mg TB-500 |
| Upper (20 units) | 7 mg | 0.2 mL | 20 units | ~5.0 mg GHK-Cu + 1.0 mg BPC-157 + 1.0 mg TB-500 |
Timing & cycling
Flexible AM or PM; inject subcutaneously into abdominal fat and rotate sites.
Cycle length. 4-8 weeks on, 2-4 weeks off (copper load from GHK-Cu argues against continuous use).
Storage & handling
Keep the lyophilized (unmixed) vial refrigerated at 2–8 °C (36–46 °F) in a dry, dark place — it tolerates short periods at room temperature during shipping. Once reconstituted, store it at 2–8 °C, never frozen, and use it within about 2–4 weeks (sooner if the solution turns cloudy or discolored). Swab the stopper before every draw and use a fresh needle each time.
Supplies you'll need
- GLOW vials — about 6 for an 8-week run at the upper dose
- Bacteriostatic water — 2 mL per vial (a 10–30 mL multi-dose bottle covers several vials)
- U-100 insulin syringes (0.3–0.5 mL / 30–50 unit barrels are easiest to read) — roughly 56 for 8 weeks, plus spares
- Alcohol swabs — one per injection and one per vial draw
- Sharps container for safe needle disposal
Key points
- Doses are given as total-blend mg; the GHK-Cu:BPC-157:TB-500 ratio is fixed at 5:1:1 and cannot be tuned per component.
- Reconstituting 70 mg in 2 mL gives clean 35 mg/mL math (1 unit = 0.35 mg total blend).
- GHK-Cu is ~71% of the blend, so most of each dose is copper peptide - keep cycles bounded and take washouts.
- No blend-level human trials exist; evidence is extrapolated from the separate GHK-Cu, BPC-157 and TB-500 pages.
- Keep the lyophilized vial cold before mixing and refrigerate after reconstitution.
What to monitor
Injection-site tolerance and signs of copper excess (nausea, metallic taste, unusual fatigue).
Legal & prescription status
No - sold for research use only; not FDA-approved.
References
This guide is for research and educational purposes only. It is not medical advice and does not describe a product approved for human use. Always consult a qualified healthcare professional before considering any peptide protocol.