Thymosin Alpha-1 Dosage & Reconstitution Guide
⚠ Not FDA-approved in the US; research-grade product is 'for research use only.' Because it modulates the immune system, avoid or use only under medical guidance if you have an autoimmune condition, are immunosuppressed or a transplant recipient, or are pregnant or breastfeeding.
Research and educational use only — not medical advice, and not for human consumption. The figures below are summarized from public sources, product labeling, and community reports, and are not a prescription or a substitute for a qualified clinician.
Reconstitution
Thymosin Alpha-1 ships as a freeze-dried (lyophilized) powder that has to be mixed with bacteriostatic water before use. Draw 2 mL of bacteriostatic water into a syringe and add it to a 10 mg vial, letting the stream run down the inside wall of the glass rather than blasting the powder directly. Swirl gently — never shake — until the solution is clear, then keep it refrigerated.
Worked example. 2 mL into a 10 mg vial gives a concentration of 5 mg/mL (5000 mcg/mL). A 1.6 mg dose then works out to 0.32 mL = 32 units on a U-100 insulin syringe. Scale the water volume up or down to make your target dose land on an easy-to-read mark (1 unit = 0.01 mL).
- Wipe both vial stoppers (peptide and bacteriostatic water) with an alcohol swab and let them dry.
- Draw 2 mL of bacteriostatic water into a syringe.
- Inject it slowly against the inside wall of the peptide vial.
- Swirl gently until fully dissolved; do not shake.
- Label the vial with the contents, concentration, and date, and store it in the refrigerator.
Dosing schedule
| Phase | Dose | Volume | Insulin units (U-100) | Notes |
|---|---|---|---|---|
| Standard maintenance (Zadaxin) | 1.6 mg | 0.32 mL | 32 units | Twice weekly, 3-4 days apart = 32 units. Approved regimen for hepatitis B and immune-adjuvant use. |
| Daily short course (research/community) | 1.6 mg | 0.32 mL | 32 units | Once daily (32 units) for a brief acute-support block, then step down. Anecdotal, not from the label. |
Timing & cycling
Space the two weekly doses 3-4 days apart; time of day is not critical. Inject subcutaneously (abdomen or thigh) and rotate sites. Store the reconstituted vial refrigerated and discard after ~28 days.
Cycle length. Approved hepatitis courses run 6-12 months; community immune-support blocks are usually 4-12 weeks, sometimes repeated seasonally.
Storage & handling
Keep the lyophilized (unmixed) vial refrigerated at 2–8 °C (36–46 °F) in a dry, dark place — it tolerates short periods at room temperature during shipping. Once reconstituted, store it at 2–8 °C, never frozen, and use it within about 2–4 weeks (sooner if the solution turns cloudy or discolored). Swab the stopper before every draw and use a fresh needle each time.
Supplies you'll need
- Thymosin Alpha-1 vials — about 3 for an 8-week run at the upper dose
- Bacteriostatic water — 2 mL per vial (a 10–30 mL multi-dose bottle covers several vials)
- U-100 insulin syringes (0.3–0.5 mL / 30–50 unit barrels are easiest to read) — roughly 16 for 8 weeks, plus spares
- Alcohol swabs — one per injection and one per vial draw
- Sharps container for safe needle disposal
Key points
- Distinct from 'thymalin' (a different, multi-peptide thymic-extract preparation) and from thymosin beta-4 / TB-500 (a repair peptide): Ta1 is a single defined 28-amino-acid immune-signaling peptide.
- It modulates rather than only boosts: it can up-regulate weak immune responses and help restrain over-activation.
- Strongest human evidence is in chronic hepatitis B, its approved indication; sepsis and general immune-support uses are far less certain after the large negative TESTS phase 3 trial.
- Reconstitute with bacteriostatic water; the mixed solution should be refrigerated (2-8 C) and used within about 28 days.
- US research-grade product is not a regulated medicine, so purity, sterility and dosing accuracy are not guaranteed.
What to monitor
Injection-site tolerance and any flu-like or new autoimmune symptoms. In hepatitis use, prescribers track liver enzymes (ALT/AST) and viral markers.
Legal & prescription status
Prescription medicine where licensed (as Zadaxin / thymalfasin in ~30 countries). Not FDA-approved in the US, where it is sold only as a research chemical labeled 'not for human consumption.'
References
This guide is for research and educational purposes only. It is not medical advice and does not describe a product approved for human use. Always consult a qualified healthcare professional before considering any peptide protocol.