SS-31 Dosage & Reconstitution Guide
Research and educational use only — not medical advice, and not for human consumption. The figures below are summarized from public sources, product labeling, and community reports, and are not a prescription or a substitute for a qualified clinician.
Reconstitution
SS-31 ships as a freeze-dried (lyophilized) powder that has to be mixed with bacteriostatic water before use. Draw 1 mL of bacteriostatic water into a syringe and add it to a 10 mg vial, letting the stream run down the inside wall of the glass rather than blasting the powder directly. Swirl gently — never shake — until the solution is clear, then keep it refrigerated.
Worked example. 1 mL into a 10 mg vial gives a concentration of 10 mg/mL (10000 mcg/mL). A 5 mg dose then works out to 0.5 mL = 50 units on a U-100 insulin syringe. Scale the water volume up or down to make your target dose land on an easy-to-read mark (1 unit = 0.01 mL).
- Wipe both vial stoppers (peptide and bacteriostatic water) with an alcohol swab and let them dry.
- Draw 1 mL of bacteriostatic water into a syringe.
- Inject it slowly against the inside wall of the peptide vial.
- Swirl gently until fully dissolved; do not shake.
- Label the vial with the contents, concentration, and date, and store it in the refrigerator.
Dosing schedule
| Phase | Dose | Volume | Insulin units (U-100) | Notes |
|---|---|---|---|---|
| Week 1 | 2.5 mg | 0.25 mL | 25 units | once daily |
| Weeks 2–8 | 5 mg | 0.5 mL | 50 units | once daily |
Timing & cycling
Morning dosing is common; no food dependency.
Cycle length. 8 weeks is the block most users run before reassessing.
Storage & handling
Keep the lyophilized (unmixed) vial refrigerated at 2–8 °C (36–46 °F) in a dry, dark place — it tolerates short periods at room temperature during shipping. Once reconstituted, store it at 2–8 °C, never frozen, and use it within about 2–4 weeks (sooner if the solution turns cloudy or discolored). Swab the stopper before every draw and use a fresh needle each time.
Supplies you'll need
- SS-31 vials — about 28 for an 8-week run at the upper dose
- Bacteriostatic water — 1 mL per vial (a 10–30 mL multi-dose bottle covers several vials)
- U-100 insulin syringes (0.3–0.5 mL / 30–50 unit barrels are easiest to read) — roughly 56 for 8 weeks, plus spares
- Alcohol swabs — one per injection and one per vial draw
- Sharps container for safe needle disposal
Key points
- Clinical trials (elamipretide) used daily subcutaneous dosing in this range for mitochondrial disease.
- Expensive per mg — budget the full 8-week course before starting.
- Community reports cluster at 5–10 mg/day; the FDA-approved elamipretide dose (Barth syndrome) is 40 mg/day. Starting at 2.5–5 mg is the cautious end.
What to monitor
Subjective energy/endurance plus resting heart rate are the practical markers.
Legal & prescription status
No legal prescription exists. It is an unapproved research chemical, sold only for laboratory research and labelled 'not for human consumption.' It is not FDA-approved for any human use.
References
This guide is for research and educational purposes only. It is not medical advice and does not describe a product approved for human use. Always consult a qualified healthcare professional before considering any peptide protocol.