GLP-1 Dosage & Reconstitution Guide
Research and educational use only — not medical advice, and not for human consumption. The figures below are summarized from public sources, product labeling, and community reports, and are not a prescription or a substitute for a qualified clinician.
Reconstitution
GLP-1 ships as a freeze-dried (lyophilized) powder that has to be mixed with bacteriostatic water before use. Draw 2 mL of bacteriostatic water into a syringe and add it to a 10 mg vial, letting the stream run down the inside wall of the glass rather than blasting the powder directly. Swirl gently — never shake — until the solution is clear, then keep it refrigerated.
Worked example. 2 mL into a 10 mg vial gives a concentration of 5 mg/mL (5000 mcg/mL). A 2.4 mg dose then works out to 0.48 mL = 48 units on a U-100 insulin syringe. Scale the water volume up or down to make your target dose land on an easy-to-read mark (1 unit = 0.01 mL).
- Wipe both vial stoppers (peptide and bacteriostatic water) with an alcohol swab and let them dry.
- Draw 2 mL of bacteriostatic water into a syringe.
- Inject it slowly against the inside wall of the peptide vial.
- Swirl gently until fully dissolved; do not shake.
- Label the vial with the contents, concentration, and date, and store it in the refrigerator.
Dosing schedule
| Phase | Dose | Volume | Insulin units (U-100) | Notes |
|---|---|---|---|---|
| Weeks 1–4 | 0.25 mg | 0.05 mL | 5 units | weekly — starter step |
| Weeks 5–8 | 0.5 mg | 0.1 mL | 10 units | weekly |
| Weeks 9–12 | 1 mg | 0.2 mL | 20 units | weekly |
| Weeks 13–16 | 1.7 mg | 0.34 mL | 34 units | weekly, if needed |
| Week 17+ | 2.4 mg | 0.48 mL | 48 units | weekly maximum |
Timing & cycling
Same day each week, with or without food. If a dose is 5+ days late, restart the ramp rather than doubling.
Cycle length. Open-ended in clinical use; the ramp exists to manage nausea, not to reach a finish line. Escalate only when the current step is tolerated AND has stopped producing progress.
Storage & handling
Keep the lyophilized (unmixed) vial refrigerated at 2–8 °C (36–46 °F) in a dry, dark place — it tolerates short periods at room temperature during shipping. Once reconstituted, store it at 2–8 °C, never frozen, and use it within about 2–4 weeks (sooner if the solution turns cloudy or discolored). Swab the stopper before every draw and use a fresh needle each time.
Supplies you'll need
- GLP-1 vials — about 2 for an 8-week run at the upper dose
- Bacteriostatic water — 2 mL per vial (a 10–30 mL multi-dose bottle covers several vials)
- U-100 insulin syringes (0.3–0.5 mL / 30–50 unit barrels are easiest to read) — roughly 8 for 8 weeks, plus spares
- Alcohol swabs — one per injection and one per vial draw
- Sharps container for safe needle disposal
Key points
- Semaglutide-pattern schedule shown; the same ramp logic applies across GLP-1 agonists but the numbers differ by compound.
- Personal/family history of medullary thyroid carcinoma or MEN2 is a hard contraindication in the approved labeling.
- Muscle loss is the quiet cost of fast loss — lift and eat protein.
- Many people never need the top steps; the right dose is the lowest one that works.
What to monitor
Weight weekly, plus attention to hydration and protein intake. Severe abdominal pain = stop and get seen (pancreatitis/gallbladder).
Legal & prescription status
Yes. This is an FDA-approved prescription medication (GLP-1 receptor agonists such as semaglutide (Ozempic, Wegovy) and liraglutide (Saxenda)), so a legitimate, human-grade version is available only through a licensed prescriber and pharmacy. The lookalike 'research chemical' versions sold online are unapproved, not quality-controlled, and labelled 'not for human consumption.'
References
This guide is for research and educational purposes only. It is not medical advice and does not describe a product approved for human use. Always consult a qualified healthcare professional before considering any peptide protocol.